Direct detection of Mycobacterium tuberculosis lipid antigens by thin-layer chromatography |
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| Authors: | David E. Minnikin Malin Ridell James H. Parlett Robert C. Bolton |
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| Affiliation: | Department of Organic Chemistry, The University, Newcastle-upon-Tyne, U.K.;Department of Medical Microbiology, University of Gothenburg, Gothenburg, Sweden |
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| Abstract: | Abstract Free lipids were extracted from Mycobacterium tuberculosis H37Rv, and their antigenicity was assessed directly on thin-layer chromatograms (TLC) by an immunostaining technique. A family of glycolipids, composed of trehalose acylated with multimethyl branched long-chain fatty acids, was investigated. The most polar of these glycolipids was identified as a possible specific surface antigen. A pair of novel polar glycolipids also showed positive antigenic reactions. |
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| Keywords: | Mycobacterium tuberculosis Lipid antigen Antigen |
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