Regeneration and frequency of tetraploid variants of Cucumis metuliferus are affected by explant induction on semi-solid medium versus the liquid/ membrane system |
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Authors: | J Adelberg |
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Institution: | (1) Department of Horticulture, College of Agriculture, Forestry and Life Sciences, E-142 Poole Agricultural Center, Box 340375, Clemson SC 29634-0375, USA, US |
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Abstract: | Ninety-eight percent of Cucumis metuliferus (PI 482439) cotyledons regenerated shoot buds after 5 weeks on basal Murashige and Skoog medium amended with 10 μm benzyladenine. Regeneration rates of explants maintained only on agar-gelled medium versus explants induced first on a liquid/membrane
system were compared after weekly transfers of tissue from liquid/membrane to agar during the first 5 weeks of regeneration.
The number of regenerant buds increased from six per cotyledon (on agar-gelled medium) to nine per cotyledon when explants
induced on the liquid/membrane system for 3 or 4 weeks were transferred to agar-gelled medium. Shoot development, rooting
and survival in the greenhouse were adequate, regardless of whether regeneration was initiated on the agar or liquid/membrane
system. Tetraploid regenerants accounted for 9% of the 391 regenerants screened. Pollen morphology was a reliable screening
technique to identify tetraploid plants. The frequency of tetraploid plants varied from 13.5% to 6.9% after 5 weeks of induction
on the agar or liquid/membrane system, respectively. This frequency decreased to 1.5% if the explants were transferred from
the liquid/membrane system to agar after the first week of induction. Transfers during this period play a critical role in
eliminating tetraploid variants.
Received: 17 June 1996 / Revision received: 27 August 1996 / Accepted: 10 March 1997 |
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Keywords: | Cucumis spp Explant induction Agar system Liquid membrane system Tetraploid regenerants |
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