Single, chemically defined sporulation medium for Bacillus subtilis: growth, sporulation, and extracellular protease production. |
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Authors: | J H Hageman G W Shankweiler P R Wall K Franich G W McCowan S M Cauble J Grajeda and C Quinones |
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Abstract: | The composition and application of a single, chemically defined medium or growth and sporulation of Bacillus subtilis is described. At 37 degrees C cells grew with a doubling time of about 40 min; cultures attained near-maximal spore formation (70 to 80% by 12 h after the end of exponential growth and produced 1 X 10(9) to 2 X 10(9) heat-resistant free spores at 24 h. Dipicolinic acid production was completed between 7 and 11 h. Cells grown in the single, chemically defined medium excreted levels of serine and neutral proteases comparable to those excreted in nutrient broth medium. |
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