An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus |
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| Authors: | Edgar Narvaez Jorge Berendsen Fanny Guzmán José A Gallardo Luis Mercado |
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| Institution: | 1. Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research, Department of Oceanography, University of Concepción, Concepción, Chile;2. Cargill Center of Global Innovation, Camino a Pargua km 57, Colaco km 5, Calbuco, Chile;1. Programa de Doctorado en Ciencias de la Acuicultura, Universidad Austral de Chile, Los Pinos s/n, Balneario Pelluco, Puerto Montt, Chile;2. Instituto de Acuicultura, Universidad Austral de Chile, Los Pinos s/n, Balneario Pelluco, Puerto Montt, Chile;3. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Independencia 641, Valdivia, Chile |
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| Abstract: | Antimicrobial peptides (AMPs) are a pivotal component of innate immunity in lower vertebrates. The aim of this study was to develop an immunological method for quantifying AMPs in Salmo salar skin mucus. A known antimicrobial peptide derived from histone H1 previously purified and described from S. salar skin mucus (SAMP H1) was chemically synthesized and used to obtain antibodies for the quantification of the molecule via ELISA. Using skin mucus samples, a correlation of bacterial growth inhibition versus SAMP H1 concentration (ELISA) was established. The results provide the first evidence for quantifying the presence of active AMPs in the skin mucus of S. salar through the use of an immunological method. |
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