Phagocytosis by B-cells and neutrophils in Atlantic salmon (Salmo salar L.) and Atlantic cod (Gadus morhua L.) |
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| Authors: | Hanne S Øverland Eirin Fausa Pettersen Anita Rønneseth Heidrun I Wergeland |
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| Institution: | 1. Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, 784 Wilson Road, East Lansing, MI 48824, USA;2. Department of Fisheries and Wildlife, College of Agriculture and Natural Resource, Michigan State University, 480 Wilson Road, East Lansing, MI 48824, USA;3. Department of Aquatic Health Sciences, Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA;1. Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Campus Isla Teja S/N, Valdivia, Chile;2. Interdisciplinary Center for Aquaculture Research (INCAR), Víctor Lamas 1290, PO Box 160-C, Concepción, Chile;3. Veterquímica, Lonquén10.387, Maipú, Santiago Chile;1. Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-0880, Japan;2. Laboratory of Fish Immunology, Institute of Infectology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany;3. Laboratory of Comparative Immunology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-0880, Japan;4. Department of Bioscience and Biotechnology, Graduate School of Bioresourse and Bioenvironment, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan;5. National Research Institute of Aquaculture, Fisheries Research Agency, Minami-ise, Mie 516-0193, Japan;1. Guangdong Provincial Key Laboratory for Health and Safe Aquaculture, College of Life Science, South China Normal University, Guangdong 510031, PR China;2. Department of Environmental and Aquatic Animal Health, Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062-1346, USA;3. Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, The Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qindao, PR China |
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| Abstract: | Phagocytosis by fish cells has mostly been studied using adherent leucocytes, excluding suspended cells such as the majority of B-cells and neutrophils, but a recent study describes professional phagocytosis of latex beads and bacteria by B-cells from rainbow trout. In the present study, phagocytosis by B-cells and neutrophils from salmon and cod was studied. Leucocytes were isolated from peripheral blood (PBL) and head kidney (HKL). By flow cytometry analyses, proportions of MAb labelled cell populations with internalized fluorescent beads, as well as the number of beads within each cell, could be determined. Phagocytic capacity and ability were demonstrated in B-cells and neutrophils from salmon and cod. In salmon, B-cells had higher phagocytic ability than neutrophils in HKL, but not in PBL. For cod the phagocytic ability of B-cells were lower than for neutrophils in both HKL and PBL, but the phagocytic capacity of cod B-cells were higher than for neutrophils in both HKL and PBL. For salmon B-cells the phagocytic capacity was lower than or similar to neutrophils in HKL and PBL. The total phagocytic ability of leucocytes was different in the species studied. The highest phagocytic ability was observed in cod, showing similar values for PBL and HKL. Salmon PBL displayed about twice the phagocytic ability of cod PBL. There seemed to be some major differences between the two fish species concerning phagocytosis. In salmon, a rather large proportion of phagocytic leucocytes were phagocytic B-cells, indicating that B-cells may have an important function in particle clearance in this species. In cod, phagocytic leucocytes in HKL and PBL were mostly neutrophils, and only a small proportion of B-cells were phagocytic, supporting the more prominent role of innate immune functions in cod neutrophils. |
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