Structure and expression of transferrin gene of channel catfish,Ictalurus punctatus |
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| Authors: | Hong Liu Tomokazu Takano Jason Abernathy Shaolin Wang Zhenxia Sha Yanliang Jiang Jeffery Terhune Huseyin Kucuktas Eric Peatman Zhanjiang Liu |
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| Affiliation: | 1. The Fish Molecular Genetics and Biotechnology Laboratory, 203 Swingle Hall, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, Auburn, AL 36849, USA;2. Fisheries College, Huazhong Agricultural University, Wuhan 430070, China;3. Key Lab For Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;1. Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, AL 36849, USA;2. United States Department of Agriculture, Agricultural Research Service, Catfish Genetics Research Unit, Stoneville, MS, USA;3. United States Department of Agriculture, Agricultural Research Service, Stuttgart National Aquaculture Research Center, Stuttgart, AR, USA;1. School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, Alabama, USA;2. Key Laboratory of Freshwater Aquatic Biotechnology and Genetic Breeding, Ministry of Agriculture, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China;3. CSIRO Oceans and Atmosphere Flagship, Hobart, Tasmania, Australia;4. National Centre for Marine Conservation and Resource Sustainability, University of Tasmania, Launceston, Australia;5. Research and Development Corporation, Gus R. Douglass Land-Grant Institute, West Virginia State University, Institute, West Virginia, USA;6. Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, China;7. Cotton Fiber Bioscience Research Unit, New Orleans, Louisiana, USA;1. The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA;2. College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China;1. State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China;2. The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA;3. Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China;1. Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile;2. Núcleo Biotecnológico de Curauma (NBC), Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile |
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| Abstract: | Transferrin is important in iron metabolism and has been reported to be involved in disease defence responses after bacterial infection. In this study, we identified, sequenced, and characterized the transferrin gene from channel catfish, Ictalurus punctatus. The catfish transferrin gene was similar to those of other vertebrate species with 17 exons and 16 introns. Sequence analysis indicated the presence of the two duplicated lobes, each containing two sub-domains separated by a cleft harboring the iron-binding site, suggesting their structural conservation. The channel catfish transferrin cDNA encodes 679 amino acids with 42–56% similarity to known transferrin genes from various species. Southern blot analysis suggested the presence of two copies of the transferrin gene in the catfish genome, perhaps arranged in a tandem fashion. The catfish transferrin gene was mapped to a catfish BAC-based physical map. The catfish transferrin gene was highly expressed in the liver, but expression was low in most other tested tissues. Transferrin expression was significantly up-regulated after infection with Edwardsiella ictaluri, the causative agent of enteric septicemia of catfish. Such induction was also found with co-injection of iron-dextran and E. ictaluri, while transferrin expression was not significantly induced with the injection of iron-dextran alone. |
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