Functional immune response in Pecten maximus: Combined effects of a pathogen-associated molecular pattern and PAH exposure |
| |
| Authors: | Marie L. Hannam Shaw D. Bamber Tamara S. Galloway A. John Moody Malcolm B. Jones |
| |
| Affiliation: | 1. Ecotoxicology and Stress Biology Research Centre, School of Biomedical and Biological Sciences, University of Plymouth, Drake Circus, Plymouth, Devon PL4 8AA, UK;2. IRIS-Biomiljø, Mekjarvik 12, 4070 Randaberg, Norway;3. School of Biosciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, Exeter, Devon EX4 4PS, UK;4. Marine Institute, Portland Square, University of Plymouth, Drake Circus, Plymouth, Devon PL4 8AA, UK;1. Dalian Ocean University, Dalian 116023, China;2. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;1. Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell''Università 16, 35020 Legnaro, PD, Italy;2. Instituto de Investigaciones Marinas (IIM), CSIC, C/Eduardo Cabello 6, 36208 Vigo, Pontevedra, Spain;1. School of Life Sciences, Ludong University, Yantai 264025, PR China;2. Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China;1. Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry - LABCAI, Federal University of Santa Catarina, UFSC, Florianópolis, SC, Brazil;2. Aquaculture Pathology Research Center - NEPAQ, Federal University of Santa Catarina, UFSC, Florianópolis, Brazil;3. Bioinformatics Laboratory, Cell biology, Embryology and Genetics Department, Federal University of Santa Catarina, Florianópolis, Brazil;4. Laboratory of Marine Organic Chemistry – LABQOM, Oceanographic Institute, University of São Paulo, USP, São Paulo, SP, Brazil;1. Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Tunisia;3. Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, Spain |
| |
| Abstract: | Pathogen-associated molecular patterns (PAMPs) enable recognition of structures present in microorganisms such as lipopolysaccharides (LPS). LPS are an essential constituent of the outer membrane of Gram-negative bacteria, stimulating the innate immune system of invertebrates. Here, LPS from Escherichia coli (055:B5) were used to investigate the functional immune response of Pecten maximus after stimulation with a PAMP and to determine the combined effect of a phenanthrene exposure and LPS challenge. Organisms were exposed to 200 μg l?1 phenanthrene and after 7 d were injected with either physiological saline (injection controls) or LPS solution, and returned to their respective exposure tanks. Haemolymph was sampled from the scallops 48 h post-injection and immune function was assessed using a combination of cellular biological responses. The LPS challenge significantly altered the immune response in P. maximus with increased cell counts and phagocytic activity. An immunosuppressive effect of phenanthrene was also observed in this study; however, exposure to phenanthrene did not significantly impair the organism's ability to respond to a PAMP challenge. The overall level of phagocytosis and cytotoxic capability following the LPS challenge was lower in phenanthrene exposed scallops and may have consequences for disease resistance in this commercially-exploited species. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
