Catabolism of capped (3'-5')- and (2'-5')-adenylates in rat liver nuclei |
| |
| Authors: | W Michels E Schlimme |
| |
| Institution: | Laboratorium für Biologische Chemie im Fachgebiet, Organische Chemie der Universität (GH), Warburger Straβe 100, D-4790 Paderborn, FRG |
| |
| Abstract: | We describe studies concerning the ability of a nuclear dinucleoside triphosphatase to act as a decapping enzyme in RNA catabolism. The enzymatic release of GMP from the Gp3A moiety was determined in the capped RNA model compounds Gp3A3'pA, Gp3A3'pA-isoprop and Gp3A2'pA in isolated rat liver nuclei; i.e., in the environment in which the dinucleoside triphosphatase operates in vivo. The Gp3A cap moiety is hydrolyzed in (3'-5') linked nucleotides only, whereas an extension of the Gp3A in the 2'-direction prevents the nuclear triphosphatase to operate. |
| |
| Keywords: | 5′-Capped (3′-5′)-adenylate 5′-Capped (2′-5′)-adenylate Capped RNA fragment Decapping activity Nuclear dinucleoside triphosphatase RNA catabolism RNA recycling Rat liver nucleus |
| 本文献已被 ScienceDirect 等数据库收录！ |
