Conversion of D-carnitine into L-carnitine with stereospecific carnitine dehydrogenases |
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Authors: | Henning Hanschmann Hans-Peter Kleber |
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Affiliation: | (1) Institut für Biochemie, Fakultät für Biowissenschaften, Pharmazie und Psychologie, Universität Leipzig, Talstr. 33, 04103 Leipzig, Germany |
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Abstract: | D-Carnitine was converted to L-carnitine by resting and permeabilized cells as well as with purified stereospecific carnitine dehydrogenases from Agrobacterium sp. With permeabilized cells only 11% of D-carnitine was converted into L-carnitine. Using highly stereospecific D- and L-carnitine dehydrogenases from Agrobacterium sp. (pH 8.5, 50 mM D-carnitine, 1 mM NAD + , 0.1 mM NADH, 25-fold excess of L-carnitine dehydrogenase) almost 50% of the D-carnitine could be converted into L-carnitine. |
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