Analysis of protein patterns from different organs and cell fractions of trisomy 19 mice |
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Authors: | Evelyn Zeindl-Eberhart Gisela Grohé Joachim Klose |
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Institution: | (1) Institut für Humangenetik, Institut für Toxikologie und Embryonal-Pharmakologie, Freie Universität Berlin, Garystrasse 5, D-1000 Berlin 33, Federal Republic of Germany;(2) Institut für Pathologie, Medizinische Hochschule Lübeck, Ratzeburger Allee 160, D-2400 Lübeck, Federal Republic of Germany |
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Abstract: | Summary Proteins were extracted from liver, brain, and skin of 6-day-old mice with trisomy (Ts) 19 and fractionated into solubilized cell proteins and structure-bound cell proteins. The proteins were separated by two-dimensional electrophoresis, and protein patterns were compared in the combinations Ts/normal and normal/normal. Analysis of the protein patterns revealed protein spots (variants) with densities higher (h-type) or lower (l-type) in trisomies than in normal mice. Some of these variants were found in all Ts individuals investigated for a particular protein class. These variants, termed regular Ts-variants, constituted 0.8%–1.6% of the total number of spots. The proteins of the regular Ts variants were in most cases organ-nonspecific. However, in almost all cases a given quantitative variation was expressed in only one of the three organs investigated. To explain our results, we have presented models for the control of protein levels on the basis of gene regulation. New aspects in the conception of studies on trisomies in man could be gained. |
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