Molecular cloning of the 5-aminolevulinic acid dehydratase gene from Rhodobacter sphaeroides. |
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| Authors: | A M Delaunay C Huault and A P Balang |
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| Institution: | Centre National de la Recherche Scientifique, Unité Associée 203, Faculté des Sciences, Mont Saint Aignan, France. |
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| Abstract: | A hemB mutant of Escherichia coli was used to clone the gene encoding 5-aminolevulinic acid dehydratase from Rhodobacter sphaeroides after physiological complementation of the mutation. A 2.9-kb DNA fragment was obtained and cloned in both orientations into the unique PstI restriction site of pUC19. This recombinant plasmid encodes a protein (Mr 39,000) that is immunoreactive with antibodies raised against the enzyme from higher plants. |
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