Effects of medium composition on hirudin production in recombinant Saccharomyces cerevisiae |
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Authors: | Chi-Min Choi Myoung-Dong Kim Sang-Ki Rhee Jin-Ho Seo |
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Institution: | (1) Department of Food Science & Technology, Research Center for New Bio-Materials in Agriculture, Seoul National University, Seoul, Korea;(2) Korea Research Institute of Bioscience & Biotechnology, Korea |
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Abstract: | Summary The enriched medium based on yeast nitrogen basc(YNB)increased hirudin synthesis and secretion in rccombinant Saccharomyces cerevisiae in batch and fed-batch cultures. Fed-batch fermentation with the defined medium yielded 342mg hirudin/l but supplementation of yeast extract increased the final hirudin concentration to 461mg hirudin/l. The defined medium, however, produced the product protein with higher purity of 21% and hence will allow easy separation of secreted hirudin from other contaminated polypeptides present in the growth medium. In a continuous culuture, the defined medium yielded higher concentrations of cell mass and hirudin than the complex medium. |
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