T and B cell responses to cytomegalovirus antigens in healthy blood donors and bone marrow transplant recipients |
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| Authors: | Francesco Lolli Vivi-Anne Sundqvist Antonella Castagna Per Ljungman Annika Linde Gudrun Andersson Tomas Olsson Britta Wahren |
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| Affiliation: | Department of Virology, National Bacteriological Laboratory, Solna, Stockholm, Sweden;Department of Neurology, Karolinska Institute, Huddinge Hospital, Huddinge, Sweden;Department of Medical Laboratory Technology, College of Health and Caring Sciences, Stockholm, Sweden;Department of Internal Medicine, Huddinge Hospital, Huddinge, Sweden;Kabi Biopharma, R&D Immunobiology, Stockholm, Sweden |
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| Abstract: | Abstract We measured the production of interferon-gamma (IFN-γ) from single T cells and the T cell proliferative response to different cytomegalovirus (CMV) antigens in healthy blood donors and bone marrow transplant recipients. The antigens consisted of a CMV nuclear antigen (CMV na) containing the pp65-kDa matrix protein and the immediate early antigens but lacking CMV glycoproteins, and an antigen comprising native CMV glycoproteins (CMV gp). We also measured the IgG antibodies to CMV na and CMV gp. The T cells reacted to CMV na in CMV seropositive blood donors both with the production of IFN-γ and with proliferation, while bone marrow transplant recipients had a deficient T cell response. After stimulation with CMV gp, no T cell response could be observed in CMV seropositive subjects. IgG antibodies to CMV na coexisted in plasma with similar levels of antibodies to CMV gp. |
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| Keywords: | Cytomegalovirus T cell B cell Interferon type II Bone marrow transplantation |
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