A Dioxan Technic for Triple Staining

The first part of the paper summarizes the ways in which technicians have used dioxan in the various phases of microtechnic. The rest of the paper consists of a detailed account of the use of dioxan in triple staining. Specific directions are given for modifications of Castroviejo's method and Mallory's triple connective tissue stain. Special emphasis is placed upon the fact that dioxan will not wash the stains out of the sections in the dehydrating process to any great extent. This makes triple staining easier.