| High efficiency DNA delivery into swine oocytes and embryos by electronic pulse delivery （EPD） |
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| 引用本文： | YANGYANG SHAOHUAHUANG 等.High efficiency DNA delivery into swine oocytes and embryos by electronic pulse delivery （EPD）[J].细胞研究,1997,7(1):39-49. |
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| 作者姓名： | YANGYANG SHAOHUAHUANG |
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| 作者单位： | [1]InCell,Inc.2310WalshAvenue,SantaClara,California95051,USA. [2]InCell,Inc.2310WalshAvenue,SantaClara,Cali |
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| 摘 要： | The production of transgenic swine for xenotransplantation has been proposed as an optimal option to overcome the chronic shortage of human organ donors.Generation of genetically engineered swine has been elusive due to the difficulties in gene transfer.In order to achieve effective gene delivery,a key step for the genetic modification,we applied electronic pulse delivery (EPD) technology to introduce H2K^b-DC DNA construct into swine eggs.Using the developed EPD Protocols^TM,we have achieved good viability of the EPD treated oocytes,satisfactory embryonic development of the EPD treated embryos,and stable DNA transfer into the swine embryos with high efficiency.Thus,application of the EPD technology promises to effectively facilitate the generation of large trangenic mammals.
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| 关 键 词： | EPD 猪卵母细胞 高效DNA转移 电脉冲转移 转基因胚胎 器官移植供体 |
High efficiency DNA delivery into swine oocytes and embryos by electronic pulse delivery (EPD) |
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| YANG YANG, SHAO HUA HUANG, XI ZHAO.High efficiency DNA delivery into swine oocytes and embryos by electronic pulse delivery (EPD)[J].Cell Research,1997,7(1):39-49. |
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| Authors: | YANG YANG SHAO HUA HUANG XI ZHAO |
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| Abstract: | The production of transgenic swine for xenotransplantation has been proposed as an optimal option to overcome the chronic shortage of human organ donors. Generation of genetically engineered swine has been elusive due to the difficulties in gene transfer. In order to achieve effective gene delivery, a key step for the genetic modification, we applied electronic pulse delivery (EPD) technology to introduce HZKb-DC DNA construct into swine eggs. Using the developed EPD ProtocolsTM, we have achieved good viability of the EPD treated oocytes, satisfactory embryonic development of the EPD treated embryos, and stable DNA transfer into the swine embryos with high efficiency. Thus, application of the EPD technology promises to effectively facilitate the generation of large trangenic mammals. |
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| Keywords: | Xenotransplantation transgenic pig electronic pulse delivery (EPD) swine embryos DNA transfer |
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