Adhesion of plant roots to poly-L-lysine coated polypropylene substrates |
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Authors: | Towler Melissa J Weathers Pamela J |
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Institution: | Department of Biology/Biotechnology, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609-2280, USA. |
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Abstract: | The ability to immobilize plant tissue in a bioreactor is an important process tool. We have shown that roots of several species rapidly attach to poly-L-lysine coated polypropylene mesh in a liquid environment. Using transformed roots of Artemisia annua as a model, the attachment process was found to be enhanced by sheep serum, but not BSA and inhibited by excess Mn(2+), but unaffected by Ca(2+) or Mg(2+). Attempts to characterize the molecule(s) responsible for binding using lectins and antibodies showed that the binding site does not appear to be glycosylated or vitronectin-like. This method of rapid attachment should prove useful for controlled immobilization of roots in bioreactors. |
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