Stable isotope evidence for the localisation of some metabolic pathways in the bacterium Paracoccus denitrificans |
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| Authors: | R H Dunstan F R Whatley W Greenaway |
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| Institution: | Department of Plant Sciences, University of Oxford, U.K. |
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| Abstract: | Paracoccus denitrificans was grown on 6-13C]-glucose as the sole carbon source for growth and the extracts were fractionated and analysed by gas chromatography-mass spectrometry. The 13C-enrichments of some metabolites indicated that the "hydrolysate pools" of these metabolites were not in isotopic equilibrium with the water soluble "free pools". It was concluded that localisation of some metabolic pathways had occurred in Paracoccus during growth on 6-13C]-glucose. |
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