Colony aggregation and biofilm formation in xylem chemistry-based media for Xylella fastidiosa |
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Authors: | Leite Breno Andersen Peter Craig Ishida Maria Lucia |
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Affiliation: | North Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, 155 Research Road, Quincy, FL 32351, USA. bleite@mail.ufl.edu |
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Abstract: | Two chemically defined media based on xylem fluid chemistry were developed for Xylella fastidiosa. These media were tested and compared to chemically defined media XDM2, XDM4 and XF-26. New media were evaluated for the Pierce's disease (PD) strain UCLA-PD. Our media either was similar to the concentration of some amino acids found in the xylem fluid of the PD-susceptible Vitis vinifera cv. Chardonnay (medium CHARD2) or incorporated the tripeptide glutathione found in xylem fluid composition (medium 3G10-R). CHARD2 and 3G10-R are among the simplest chemically defined media available. Xylem fluid chemistry-based media supported X. fastidiosa growth and especially stimulated aggregation and biofilm formation. |
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Keywords: | Xylem fluid chemistry Glutathione Chemically defined medium Biofilm Aggregation Xylella fastidiosa |
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