Evaluation of ELISA for detection of Giardia lamblia-specific copro-antigen employing monospecific antibodies |
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| Authors: | P Dutt V K Vinayak |
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| Affiliation: | Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India. |
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| Abstract: | An enzyme linked immunosorbent assay (ELISA) system, using monospecific antibodies for the detection of Giardia lamblia specific 66 kDa copro-antigen has been developed and evaluated. The assay detected the antigen in stool eluates of all the 24 microscopically confirmed cases of giardiasis and in 17 (68%) of the 25 microscopy-negative clinically suspected cases of giardiasis. None of stool eluates from 20 subjects infected with other protozoal/helminthic intestinal parasites or from 20 apparently healthy subjects had G. lamblia-specific copro-antigen. The ELISA employing monospecific antibodies is a sensitive and specific tool for the diagnosis of giardiasis and is especially useful for confirming microscopy-negative suspected cases of giardiasis. |
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