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Crystallization of the terminal oxygenase component of biphenyl dioxygenase derived from Rhodococcus sp. strain RHA1 |
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| Authors: | Nagarajan Venugopalan Sakurai Nobuyuki Kubota Miho Nonaka Takamasa Nagumo Hiroaki Takeda Hisashi Nishizaki Tomoko Masai Eiji Fukuda Masao Mitsui Yukio Senda Toshiya |
| Institution: | Division of Protein Engineering, Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan. |
| Abstract: | The terminal oxygenase component of the biphenyl dioxygenase (BphA1A2 complex) was over-expressed with a novel over expression system in recombinant Rhodococcus strain and purified. The purified enzyme has been crystallized by the hanging drop vapor diffusion method and subjected to X-ray diffraction analysis. The crystals belong to the tetragonal system in the space group P4(1)2(1)2 or P4(3)2(1)2 and diffract to better than 2.2A resolution. |
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