Heterogeneous organization of a tandem repeat family in subtelomeric heterochromatin of rye |
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Authors: | E V Evtushenko A V Vershinin |
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Institution: | 1.Institute of Chemical Biology and Fundamental Medicine, Siberian Branch,Russian Academy of Sciences,Novosibirsk,Russia |
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Abstract: | The presence of tandem repeat multicopy families in subtelomeric regions of all chromosomes is a characteristic feature of
the rye karyotype, in contrast to the organization of these regions in chromosomes of extensively studied species, such as
human, rice, and Arabidopsis. To study the molecular structure of these regions, we analyzed BAC clones from a library constructed from the genetic material
of rye chromosome 1 short arm (1RS). Screening of the library detected numerous clones that contained copies of multicopy
tandem families of DNA sequences pSc200, pSc250, and pSc119.2. An examination of the molecular organization of tandem arrays
of the pSc200 family, which is the most common in the rye genome, showed that the subtelomeric 1RS region includes several
such arrays, each of which contains characteristic blocks of multimers of various periodicity. Such pattern of heterogeneous
organization of tandem repeat arrays differs from the view of the tandem arrays as monotonous sequence of identical monomers,
which was generally accepted in recent past. |
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