Incorporation into planar lipid bilayers of ATP-regulated K+ channels from membranes of the insulin secreting beta-cell line, HIT T15. |
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Authors: | Y Oosawa |
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Institution: | University Laboratory of Physiology, Oxford, UK. |
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Abstract: | Ion channels were incorporated into planar lipid bilayers following fusion of vesicles from the membrane of an insulin-secreting beta-cell line, HIT T15. The channel was completely blocked by 0.5 mM ATP. The channel retained the same ATP-dependence, voltage-sensitivity and single channel conductance as the ATP-regulated K+ channel that found in isolated membrane patches. |
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