Lack of germ-line promoter methylation in BRCA1-negative families with familial breast cancer |
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Authors: | Chen Ying Toland Amanda E McLennan Jane Fridlyand Jane Crawford Beth Costello Joseph F Ziegler John L |
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Institution: | Department of Neurological Surgery, UCSF Comprehensive Cancer Center, San Francisco, California 9443-0875, USA. |
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Abstract: | Hereditary breast cancer accounts for about 10% of breast cancer in the United States, but high-penetrance, germ-line mutations in BRCA1 and BRCA2 are responsible for less than half of these high-risk families. Epigenetic modification of DNA by promoter methylation can result in a potentially heritable epimutation that silences the gene. Using a highly sensitive technique, we assayed the BRCA1 gene for promoter methylation among 41 BRCA1- and BRCA2-negative women whose personal and family histories indicated a high risk of BRCA mutations (median prior likelihood = 60%) using the BRCAPro model. DNA from 19 women who were "true negatives" for BRCA mutations served as controls. We found no evidence for promoter methylation among the high-risk women who tested negative for germ-line BRCA mutations. Thus, epimutation is an unlikely explanation for hereditary breast cancer in women who test negative for BRCA mutations. |
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