Combining bulk segregation analysis and microarrays for mapping of the pH trait in melon |
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Authors: | Amir Sherman Ravit Eshed Rotem Harel-Beja Galil Tzuri Vitaly Portnoy Shahar Cohen Mor Rubinstein Arthur A Schaffer Joseph Burger Nurit Katzir Ron Ophir |
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Institution: | 1. Genomic Unit, Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250, Bet Dagan, Israel 3. Institute of Plant Sciences, Newe Ya’ar Research Center, Agricultural Research Organization, P.O. Box 1021, 30095, Ramat Yishay, Israel 2. Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250, Bet Dagan, Israel
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Abstract: | The availability of sequence information for many plants has opened the way to advanced genetic analysis in many non-model plants. Nevertheless, exploration of genetic variation on a large scale and its use as a tool for the identification of traits of interest are still rare. In this study, we combined a bulk segregation approach with our own-designed microarrays to map the pH locus that influences fruit pH in melon. Using these technologies, we identified a set of markers that are genetically linked to the pH trait. Further analysis using a set of melon cultivars demonstrated that some of these markers are tightly linked to the pH trait throughout our germplasm collection. These results validate the utility of combining microarray technology with a bulk segregation approach in mapping traits of interest in non-model plants. |
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