Improvement of the total turnover number and space-time yield for chloroperoxidase catalyzed oxidation |
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Authors: | Seelbach K van Deurzen M P van Rantwijk F Sheldon R A Kragl U |
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Institution: | Forschungszentrum Jülich GmbH, Institut für Biotechnologie, D-52425 Jülich, Germany; telephone: +49-2461-616772; fax: +49-2461-613870; email: u.kragl@kfa-juelich.de. |
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Abstract: | Chloroperoxidase from Caldariomyces fumago was applied for the oxidation of indole to oxindole using hydrogen peroxide as the oxidant in aqueous t-butyl alcohol medium. Different ways of adding the oxidant, various reactor types, and the use of a hydrogen peroxide-stat were compared, resulting in a 20-fold increase of the total turnover number (ttn) and space-time yield (sty). The highest ttn of >860,000 was obtained in a fed-batch reactor, whereas the highest sty of 120 g/(L . d) was reached in a continuously operated enzyme membrane reactor. The results were compared to other enzyme systems already established for the synthesis of amino acids and carbohydrates. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 283-288, 1997. |
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