Transcriptional regulation of the human thromboxane A2 receptor gene by Wilms' tumor (WT)1 and hypermethylated in cancer (HIC) 1 in prostate and breast cancers |
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| Authors: | Garret L. Keating Helen M. Reid Sarah B. Eivers Eamon P. MulvaneyB. Therese Kinsella |
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| Affiliation: | UCD School of Biomolecular and Biomedical Sciences, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield Dublin 4, Ireland |
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| Abstract: | The prostanoid thromboxane (TX) A2 plays a central role in hemostasis and is increasingly implicated in neoplastic disease, including prostate and breast cancers. In humans, TXA2 signals through the TPα and TPβ isoforms of the T prostanoid receptor, two structurally related receptors transcriptionally regulated by distinct promoters, Prm1 and Prm3, respectively, within the TP gene. Focusing on TPα, the current study investigated its expression and transcriptional regulation through Prm1 in prostate and breast cancers. |
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| Keywords: | ChIP, chromatin immunoprecipitation FBS, fetal bovine serum HEL, human erythroleukemia HRP, horse radish peroxidase IHC, immunohistochemistry PBS, phosphate-buffered saline pGL3B, pGL3Basic PRK, protein kinase C-related kinase pRL-TK, pRL-thymidine kinase qRT-PCR, quantitative reverse-transcriptase-polymerase chain reaction RLU, relative luciferase unit RR, repressor region SDM, site-directed mutagenesis TP, thromboxane A2 receptor TX, thromboxane TXS, TXA synthase UAR, upstream activator region URR, upstream repressor region. |
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