Manganese elevates manganese superoxide dismutase protein level through protein kinase C and protein tyrosine kinase |
| |
Authors: | Sufen Li Lin Lu Xiudong Liao Tianquan Gao Funing Wang Liyang Zhang Lin Xi Songbai Liu Xugang Luo |
| |
Affiliation: | 1.Mineral Nutrition Research Division, Institute of Animal Science,Chinese Academy of Agricultural Sciences (CAAS),Beijing,People’s Republic of China;2.Department of Animal Science,Hebei Normal University of Science and Technology,Qinhuangdao,People’s Republic of China;3.Department of Animal Science,North Carolina State University,Raleigh,USA;4.Key Laboratory of Animal Nutrition and Feed Science of the Ministry of Agriculture,Guangdong Wen’s Foodstuffs Group Corporation Ltd.,Yunfu,People’s Republic of China |
| |
Abstract: | Three experiments were conducted to investigate the effects of inorganic and organic Mn sources on MnSOD mRNA, protein and enzymatic activity and the possible signal pathways. The primary broiler myocardial cells were treated with MnCl2 (I) or one of organic chelates of Mn and amino acids with weak, moderate (M) or strong (S) chelation strength for 12 and 48 h. Cells were preincubated with superoxide radical anions scavenger N-acetylcysteine (NAC) or specific inhibitors for MAPKs and protein tyrosine kinase (PTK) or protein kinase C (PKC) for 30 min before treatments of I and M. The MnSOD mRNA, protein and enzymatic activity, phosphorylated MAPKs or protein kinases activations were examined. The results showed that additions of Mn increased (P < 0.05) MnSOD mRNA levels and M was more effective than I. Additions of Mn elevated (P < 0.05) MnSOD protein levels and enzymatic activities, and no differences were found among I and M. Addition of NAC did not decrease (P > 0.05) Mn-induced MnSOD mRNA and protein levels. None of the three MAPKs was phosphorylated (P > 0.05) by Mn. Additions of Mn decreased (P < 0.05) the PTK activities and increased (P < 0.05) the membrane PKC contents. Inhibitors for PTK or PKC decreased (P < 0.05) Mn-induced MnSOD protein levels. The results suggested that Mn-induced MnSOD mRNA and protein expressions be not related with NAC, and MAPK pathways might not involve in Mn-induced MnSOD mRNA expression. PKC and PTK mediated the Mn-induced MnSOD protein expression. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|