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Cloning of tomato nuclear ribosomal DNA. rDNA organization in leaves and suspension-cultured cells |
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| Authors: | Boris Dobrowolski Konrad Glund and Michael Metzlaff |
| Institution: | a WB Genetik, Domplatz 1, Martin-Luther-Universität Halle-Wittenberg, DDR - 4020, Halle, G.D.R. b WB Biochemie, Pflanzenbiochemische Abteilung, Neuwerk 1, Martin-Luther-Universität Halle-Wittenberg, DDR - 4020, Halle, G.D.R. |
| Abstract: | The rDNA fragments of EcoRI digested tomato nuclear DNA were detected by cross-hybridization with cloned Aspergillus nidulans rDNA. Two fragments coding for 18S and 25S rRNA, respectively were cloned and characterized. They were used to study structural organization of rRNA genes of a tomato cell suspension culture by hybridization with specifically cleaved nuclear DNA. The repeating units found were variable in restriction sites and in size with a basic unit of 8.6 kbp. Additionally, the analysis clearly demonstrates that nuclear DNA isolated from tomato leaves (Lycopersicon esculentum cv. Lukullus) and cultured cells derived therefrom bear significant differences in the structural organization of their ribosomal DNA. |
| Keywords: | Lycopersicon esculentum suspension culture rDNA cloning physical mapping rDNA heterogeneity |
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