Bioluminescence Activity and Degradation Kinetics of Genetically Engineered Strain P. putida mt-2 RB 1401 in Soil System with m-Xylene |
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| Authors: | IN CHUL KONG T H PARK T B AHN R S BURLAGE |
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| Institution: | 1. Department of Environmental Engineering , Yeungnam University , Gyungbuk, Korea;2. Department of Civil Engineering , Hanyang University , Seoul, Korea;3. Department of Civil and Environmental Engineering , Woosong University , Daejon, Korea;4. Health Sciences Department , University of Wisconsin, Milwaukee , Gyungbuk, WI, USA |
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| Abstract: | Strain RB1401 contains a plasmid with a fusion between the upper pathway promoter of the TOL plasmid xyl genes and a lux gene cassette. Bioluminescence activity appeared in the early period of biodegradation of m-xylene, and was observed at all tested conditions of temperature (15, 27, 35°C) and pH (5, 7, 9), showing in the range of 300 ～ 6100 RLU. Biokinetics of strain RB1401 in the presence of m-xylene were as follows: Y 0.56 mg/mg, μmax 2.67 day-1, and Ks 19.25 mg/L. A ratio of C:N(KNO3):P(KH2PO4) = 100:8:1.5 and 0.01% (w/v) of H2O2 were chosen for optimum biostimulation conditions of RB1401 with m-xylene in soil system. These results demonstrate the capability of such a genetically engineered bacteria for the development of biomonitoring and bioremediation in soil environments contaminated with specific volatile organic compounds. |
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| Keywords: | Biokinetics bioluminescence activity lux gene TOL plasmid |
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