Kinetics of citrate uptake in growing cells of Leuconostoc spp. |
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Authors: | Talal Belguendouz Rémy Cachon Charles Diviès |
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Institution: | Facultéd'?nologie de Bordeaux, 351, Cours de la Libération, 33400 Talence, France; IBGC, 1, rue Camille Saint Saëns, 33077 Bordeaux, France |
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Abstract: | Abstract Several yeast strains of the species Saccharomyces cerevisiae, S. bayanus and S. paradoxus , first identified by hybridization experiments and measurements of DNA/DNA homology, were characterized using polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) analysis of the MET2 gene. There was no exception to the agreement between this method and classical genetic analyses for any of the strains examined, so PCR/RFLP of the MET2 gene is a reliable and fast technique for delimiting S. cerevisiae and S. bayanus . Enological strains classified as S., bayanus , S. chevalieri , and S. capensis gave S. cerevisiae restriction patterns, whereas most S. uvarum strains belong to S. bayanus . Enologists should no longer use the name of S. bayanus for S. cerevisiae Gal strains, and should consider S. bayanus as a distinct species. |
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Keywords: | Molecular taxonomy Saccharomyces cerevisiae Saccharomyces hayanus PCR/RFLP Electrophoretic karyotype |
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