rRNA-protein neighbourhood in Escherichia coli 70 S ribosomes and 70 S initiation complex. Probing by bifunctional Pt(II)-containing reagent |
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| Authors: | P G Chistyakov M N Abdukayumov A G Veniaminova S N Vladimirov D M Graifer S A Kazakov G G Karpova |
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| Affiliation: | Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, USSR Academy of Sciences, Novosibirsk. |
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| Abstract: | The cleavable homobifunctional reagent dichloro[N,N,N',N'-tetrakis(2-aminoethyl)-1,6-hexamethylenediamminedi platinum (II)] dichloride was used for studying rRNA-protein cross-links in free 35S-labelled 70 S ribosomes and within initiation complex ribosome.AUGU6.fMet-tRNA(fMet). It was shown that the sets of proteins cross-linked to 16 S and 23 S rRNA in free 70 S ribosomes and in 70 S initiation complex do not differ significantly. The authors are the first to demonstrate most of the 23 S rRNA-protein cross-links and some 16 S rRNA-protein cross-links, in particular those with L7/L12 protein. |
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