Purification of a high molecular weight somatomedin binding protein from human plasma |
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| Authors: | G Enberg |
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| Institution: | 1. Department of Food and Drug Science, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy;2. Department of Veterinary Science, University of Parma, Via del Taglio 10, 43100 Parma, Italy;3. Section of Dermatology, Department of Medicine and Surgery, University of Parma, Via Gramsci, 43100 Parma, Italy;1. Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, AB, Canada;2. Alberta Precision Laboratories, Calgary, AB, Canada;3. Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA |
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| Abstract: | The somatomedins insulin-like growth factor I and II (1,2) are in serum bound to high-molecular weight binding proteins (6,7,8). By use of a four step chromatographic procedure a somatomedin binding protein was isolated from outdated human plasma. Exclusion chromatography on Sephadex G-200 disclosed a molecular weight of 150 kDa. After lyophilization however, the binding activity was found in a lower molecular weight range of 35-45 kDa. A partial amino acid sequence analysis of the lyophilized material revealed a possible N-terminal sequence of Ala-Pro-Trp. This sequence is identical to the N-terminal sequence of the 35 kDa somatomedin binding protein previously isolated from human amniotic fluid (16). |
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