Role of cysteine 341 and arginine 348 of GLP-1 receptor in G-protein coupling |
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Authors: | Aljoša Bavec Yang Jiang Ülo Langel Matjaž Zorko |
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Institution: | Institute of Biochemistry, Medical Faculty, University of Ljubljana, Vrazov trg 2, 1000, Ljubljana, Slovenia. aljosa.bavec@mf.uni-lj.si |
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Abstract: | We have demonstrated the ability of peptides derived from the third intracellular loop of GLP-1 receptor to differently modulate
activity of four different types of G-proteins overexpressed in sf9 cells. In this respect, the involvement of Cys341 in inhibition of Gs and Cys341 in activation of Gs and in inhibition of Gi1, Go, and G11, respectively, indicates their potential role in discrimination between different types of G-proteins. Moreover, these two
amino acids from the third intracellular loop might represent an important novel targets for covalent modification by downstream
regulators in signaling through GLP-1 receptor. |
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Keywords: | G-protein-coupled receptors GLP-1 Glucagon-like peptide-1 receptor G proteins Immunoblotting Sf9 cells |
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