An efficient biocatalytic synthesis of imidazole-4-acetic acid |
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| Authors: | Weirui Zhao Huanru Ding Sheng Hu Jun Huang Changjiang Lv Jiaqi Mei Zhihua Jin Shanjing Yao Lehe Mei |
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| Institution: | 1.School of Biological and Chemical Engineering, Ningbo Institute of Technology,Zhejiang University,Ningbo,China;2.College of Chemical and Biological Engineering,Zhejiang University,Hangzhou,China;3.Department of Chemical Engineering,The University of Utah,Salt Lake City,USA;4.School of Biological and Chemical Engineering,Zhejiang University of Science and Technology,Hangzhou,China |
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| Abstract: | ObjectiveTo develop a new and efficient biocatalytic synthesis method of imidazole-4-acetic acid (IAA) from l-histidine (l-His).Resultsl-His was converted to imidazole-4-pyruvic acid (IPA) by an Escherichia coli whole-cell biocatalyst expressing membrane-bound l-amino acid deaminase (ml-AAD) from Proteus vulgaris firstly. The obtained IPA was subsequently decarboxylated to IAA under the action of H2O2. Under optimum conditions, 34.97 mM IAA can be produced from 50 mM l-His, with a yield of 69.9%.ConclusionsCompared to the traditional chemical synthesis, this biocatalytic method for IAA production is not only environmentally friendly, but also more cost effective, thus being promising for industrial IAA production. |
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