The use of (GACA)4 PCR to sex Old World vultures (Aves: Accipitridae) |
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| Authors: | M. WINK,H. SAUER-GÜ RTH,F. MARTINEZ,G. DOVAL,G. BLANCO,& O. HATZOFE |
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| Affiliation: | Institut für Pharmazeutische Biologie, Universität Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany, ,;Departamento de Biología Animal, Universidad de Alcaláde Henares, 28871 Alcaláde Henares, Madrid Spain, ,;Ramat Hanadiv Raptor Reintroduction Project, PO Box 133, Binyamina, Israel |
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| Abstract: | A PCR method was developed employing a single primer (GACA)4 to sex Black vultures ( Aegypius monachus ), Lappet-faced vultures ( Torgos tracheliotus ), and Griffon vultures ( Gyps fulvus ). Using the (GACA)4 primer several PCR products were generated. One or more PCR products displayed a sex-specific pattern, i.e. they were only present in females (probably corresponding to repetitive DNA on the W chromosome) but absent in males. The sex ratio of 85 Griffon vultures from Spain was almost 1. |
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| Keywords: | breeding programs molecular sexing PCR recruitment sex ratios |
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