Somatic embryogenesis from immature and mature embryos of a minor millet Paspalum scrobiculatum L. |
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Authors: | Vikrant Rashid A. |
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Affiliation: | (1) Present address: Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110 021, India;(2) Department of Botany, University of Delhi, Delhi, 110007, India |
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Abstract: | Immature and mature zygotic embryos of Paspalum scrobiculatum L. cv. PSC 1 cultured on MS or N6 nutrient medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), formed embryogenic callus. Induction of embryogenic callus and subsequent somatic embryogenesis was possible at a lower concentration of 2,4-D on N6 than MS medium. Immature embryos were highly totipotent, forming somatic embryos at a higher frequency than mature embryos. Addition of amino acids (L-proline or L-tryptophan) to 2,4-D medium resulted in significant enhancement of embryogenesis on culture of mature embryos. Silver nitrate also supported an increased frequency of embryogenesis. Thus it is possible to have high frequency of somatic embryogenesis on culture of mature embryos, which are available in abundance and with ease than immature embryos. The somatic embryos readily germinated and formed plantlets on hormone-free regeneration medium. The regenerated plantlets were successful on transfer to soil and set seed. |
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Keywords: | amino acids immature and mature embryo Paspalum scrobiculatum regeneration silver nitrate |
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