Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana |
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Authors: | Pekárová Blanka Klumpler Tomáš Třísková Olga Horák Jakub Jansen Séverine Dopitová Radka Borkovcová Petra Papoušková Veronika Nejedlá Eliška Sklenář Vladimír Marek Jaromír Zídek Lukáš Hejátko Jan Janda Lubomír |
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Affiliation: | 1. Department of Functional Genomics and Proteomics, Masaryk University, Kotlá?ská 2, CZ‐61137 Brno, Czech Republic;2. CEITEC‐Central European Institute of Technology, Masaryk University, ?erotínovo nám. 9, CZ‐60177 Brno, Czech Republic;3. National Centre for Biomolecular Research, Masaryk University, Kotlá?ská 2, CZ‐61137 Brno, Czech Republic |
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Abstract: | Multistep phosphorelay (MSP) signaling mediates responses to a variety of important stimuli in plants. In Arabidopsis MSP, the signal is transferred from sensor histidine kinase (HK) via histidine phosphotransfer proteins (AHP1–AHP5) to nuclear response regulators. In contrast to ancestral two‐component signaling in bacteria, protein interactions in plant MSP are supposed to be rather nonspecific. Here, we show that the C‐terminal receiver domain of HK CKI1 (CKI1RD) is responsible for the recognition of CKI1 downstream signaling partners, and specifically interacts with AHP2, AHP3 and AHP5 with different affinities. We studied the effects of Mg2+, the co‐factor necessary for signal transduction via MSP, and phosphorylation‐mimicking BeF3? on CKI1RD in solution, and determined the crystal structure of free CKI1RD and CKI1RD in a complex with Mg2+. We found that the structure of CKI1RD shares similarities with the only known structure of plant HK, ETR1RD, with the main differences being in loop L3. Magnesium binding induces the rearrangement of some residues around the active site of CKI1RD, as was determined by both X‐ray crystallography and NMR spectroscopy. Collectively, these results provide initial insights into the nature of molecular mechanisms determining the specificity of MSP signaling and MSP catalysis in plants. |
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Keywords: | multistep phosphorelay receiver domain CKI1 crystal structure NMR spectroscopy Arabidopsis thaliana |
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