Glycosylated F4 (K88) Fimbrial Adhesin FaeG Expressed in Barley Endosperm Induces ETEC-neutralizing Antibodies in Mice |
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Authors: | Jussi J Joensuu Mirkka Kotiaho Teemu H Teeri Leena Valmu Anna Maria Nuutila Kirsi-Marja Oksman-Caldentey Viola Niklander-Teeri |
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Institution: | (1) Department of Applied Biology, University of Helsinki, P.O. Box 27, FIN-00014 Helsinki, Finland;(2) Department of Clinical Chemistry, University of Helsinki, P.O. Box 63, FIN-00014 Helsinki, Finland;(3) VTT Technical Research Centre of Finland, P.O. Box 1000, FIN-02044, VTT, Espoo, Finland |
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Abstract: | The F4-positive enterotoxigenic Escherichia coli (ETEC) strains are a frequent cause of porcine post-weaning diarrhea. Orally administered F4 fimbriae or FaeG, the major
subunit and adhesin of F4, induce a protective mucosal immune response in F4 receptor-positive piglets. Feed plants carrying
immunogenic subunit proteins can offer great advantages for oral vaccination of domestic animals. Here, we describe high-level
endosperm-specific production (1% of total soluble proteins) of FaeG in the crop plant barley. The endoplasmic reticulum-targeted
recombinant endospermic FaeG (erFaeG) was shown to be heterogeneously glycosylated. The erFaeG showed resistance at digestive
conditions simulating piglet gastric fluid. Glycosylation did not abolish the immunogenic character of the FaeG protein, since
erFaeG was able to induce F4 fimbria-specific antibodies in mice. Biological activity of these anti-F4 antibodies was demonstrated
in vitro by blocking the attachment of the F4+ ETEC to the F4 receptors present on porcine intestinal enterocytes. |
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Keywords: | barley enterotoxigenic Escherichia coli F4 (K88) fimbriae FaeG N-glycosylation piglet |
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