Soybean somatic embryogenesis: Effects of hormones and culture manipulations |
| |
Authors: | Paul A Lazzeri David F Hildebrand Glenn B Collins |
| |
Affiliation: | (1) Department of Agronomy, University of Kentucky, 40546-0091 Lexington, KY, USA;(2) Present address: Max-Planck-Institut, D-5000 Köln 30, BRD |
| |
Abstract: | Somatic embryos were induced in cultures of immature soybean (Glycine max (L.) Merr) embryos, or isolated cotyledons on MS modified medium supplemented with NAA and 2,4-D, BAP and ABA. When NAA and 2,4-D were compared at similar concentrations (25 and 23 M), 2,4-D produced larger number of somatic embryos, however, embryogenesis efficiency was improved in media containing NAA by using higher levels (100–150 M) of the auxin. Somatic embryo morphology varied with auxin type: NAA-induced embryos more closely resembled zygotic embryos than did 2,4-D-induced embryos. Additions of BAP or ABA to auxin-containing media had either no effect or reduced embryo production, although ABA altered the morphology of 2,4-D-induced embryos. In media containing both NAA and 2,4-D, the latter was dominant in terms of embryo morphology. The effects of subculture frequency and of transfers between 2,4-D and NAA media were investigated: Subculture frequency influenced mainly the frequency of normal embryos, while preculture on 2,4-D increased subsequent embryogenesis efficiency on NAA medium but reduced the frequency of normal embryos.Abbreviations Em-2 s-1
microEinsteins per square meter per second
- NAA
-naphthalene acetic acid
- 2,4-D
2,4-dichlorophenoxy acetic acid
- ABA
abscisic acid
- BAP
benzylamino purine
This paper (No. 86-3-96), is published with the approval of the director of the Kentucky Agricultural Experiment Station. |
| |
Keywords: | soybean somatic embryogenesis hormones tissue culture embryo morphology plant regeneration |
本文献已被 SpringerLink 等数据库收录! |
|