Characterization of an ecotype of brake-fern, Pteris vittata, for arsenic tolerance and accumulation in plant biomass |
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Authors: | B. K. Sarangi T. Chakrabarti |
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Affiliation: | (1) National Environmental Engineering Research Institute, Maharashtra, India |
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Abstract: | An ecotype of brake fern (Pteris vittata) was assessed for arsenic tolerance and accumulation in its biomass under in vivo and in vitro condition; using soil, and agar-gelled Murashige and Skoog (MS) medium supplemented with different concentrations of arsenic. The plants were raised in soil amended with 100–1000 mg arsenic kg−1 soil, and MS medium was supplemented with 10–300 mg arsenic 1−1 medium using Na2HAsO4 · 7H2O. The spores and haploid gametophytic-prothalli were raised in vitro on MS medium supplemented with arsenic. The field plants showed normal growth and biomass formation in arsenic amended soil, and accumulated 1908–4700 mg arsenic kg−1 dry aerial biomass after 10 weeks of growth. Arsenic toxicity was observed above >200 mg arsenic kg−1 soil. The concentrations of arsenic accumulated in the plant biomass were statistically significant (p < 0.05). Normal plants were developed from spores and gametophyte prothalli on the MS media supplemented with 50–200 mg arsenic 1−1 medium. The in vitro raised plants were tolerant to 300 mg arsenic kg−1 of soil and accumulated up to 3232 mg arsenic kg−1 dry aerial biomass that showed better growth performance, biomass generation and arsenic accumulation in comparison to the field plants. The text was submitted by the authors in English. |
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