| Ad-IL-24对MG-63人骨肉瘤细胞抑癌效应的体内外实验 |
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| 引用本文： | 韩亚丽,缪竞诚,盛伟华,汪小华,井莹莹,单云波,刘铁连,包婉蓉,杨吉成.Ad-IL-24对MG-63人骨肉瘤细胞抑癌效应的体内外实验[J].生物工程学报,2009,25(10):1538-1545. |
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| 作者姓名： | 韩亚丽 缪竞诚 盛伟华 汪小华 井莹莹 单云波 刘铁连 包婉蓉 杨吉成 |
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| 作者单位： | 苏州大学医学部细胞与分子生物学教研室,苏州,215123 |
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| 基金项目： | 江苏省高校自然科学基础研究项目(No.08KJB310011);;苏州大学医学发展基金资助项目(No.EE134517)资助~~ |
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| 摘 要： | 本研究旨在分析腺病毒携带的IL-24基因在体内外对人骨肉瘤细胞生长抑制效应及其分子机制。将Ad-IL-24重组腺病毒感染MG-63细胞,用荧光显微镜、RT-PCR法检测IL-24在MG-63细胞中的转录和表达;MTT法、流式细胞技术和Hoechst染色法检测IL-24基因的表达对MG-63细胞的生长抑制和凋亡效应;半定量RT-PCR法检测IL-24基因的表达对MG-63细胞中的bcl-2、bax、caspase-3相关基因表达的影响。用Ad-IL-24重组腺病毒在MG-63骨肉瘤荷瘤裸鼠的瘤体内进行注射治疗,观察肿瘤生长变化,15d后处死裸鼠,摘除瘤体,称瘤重。并通过免疫组化法检测Bcl-2、Bax、Caspase-3等与细胞凋亡相关因子的表达。结果表明Ad-IL-24重组腺病毒感染MG-63细胞后,能明显抑制MG-63细胞增殖,并能通过上调细胞中bax、caspase-3和下调bcl-2基因表达,诱导细胞凋亡,呈现出典型细胞凋亡形态学变化。Ad-IL-24重组腺病毒瘤内注射MG-63裸鼠荷瘤骨肉瘤移植瘤后,能显著抑制肿瘤生长,瘤重的抑制率可达52%,免疫组化结果显示Ad-IL-24重组腺病毒能明显上调与细胞...
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| 关 键 词： | IL-24基因 腺病毒载体 MG-63人骨肉瘤细胞 肿瘤抑制 |
| 收稿时间： | 2009/5/19 0:00:00 |
Interleukin 24 inhibits growth and induces apoptosis of osteosarcoma cells MG-63 in vitro and in vivo |
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| Yali Han,Jingcheng Miao,Weihua Sheng,Xiaohua Wang,Yingying Jing,Yunbo Shan,Tielian Liu,Wanrong Bao and Jicheng Yang.Interleukin 24 inhibits growth and induces apoptosis of osteosarcoma cells MG-63 in vitro and in vivo[J].Chinese Journal of Biotechnology,2009,25(10):1538-1545. |
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| Authors: | Yali Han Jingcheng Miao Weihua Sheng Xiaohua Wang Yingying Jing Yunbo Shan Tielian Liu Wanrong Bao and Jicheng Yang |
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| Institution: | Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China;Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China;Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China;Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China;Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China;Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China;Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China;Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China;Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China |
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| Abstract: | To study the inhibitory effect and anti-cancer mechanisms of interleukin 24 (IL-24) on human osteosarcoma cel1 MG-63, we delivered IL-24 into MG-63 cells in vitro and in vivo by adenovirus. The expression level of IL-24 was detected by RT-PCR and fluorescence microscope; the growth inhibition, apoptosis rate and apoptosis body were measured by MTT, Flow cytometry and Hoechst staining respectively. Furthermore, we analyzed the expression of bcl-2, bax, caspase3 genes by RT-PCR after overexpression of IL-24. ... |
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| Keywords: | IL-24 gene adenovirus vector human osteosarcoma cells tumor-suppression |
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