| 植物的MAR及其对转基因表达的效应 |
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| 引用本文： | 赵艳,高振宇,黄大年. 植物的MAR及其对转基因表达的效应[J]. 遗传, 2001, 23(3): 281-284 |
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| 作者姓名： | 赵艳 高振宇 黄大年 |
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| 作者单位： | 1.浙江大学生命科学学院，杭州 310012；2.杭州商学院生物工程系，杭州 310035；3.中国水稻研究所农业部水稻生物学重点实验室，杭州 3100061.College of Life Science, Zhejiang University, Hangzhou 310012, China;2.iological Engeneering Department,Hangzhou Institute of Commerce, Ha ngzhou 310035,China;3.Key Laboratory for Rice Biology,Ministry of Agriculture,China National Rice Institute,angzhou 310006,China |
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| 摘 要： | 与核基质结合的DNA序列称为基质附着区（matrix attachment regions，MARs），可提高转基因的表达水平并降低转基因在不同转基因系间的表达差异，因其在植物基因工程中的巨大应用潜力而引起了研究者的极大兴趣。对植物中MAR的研究尚处于早期阶段，本文综述了植物中MAR的分离鉴定、序列特征及MAR对植物中转基因表达的影响，并进一步讨论了MAR对转基因效应的可能机制。Abstract：DNA sequences called matrix attachment regions (MARs) have recently attracted mu ch attention because of their perceived capacity to increase levels of transgene expression and to reduce transformant-to-transformant variation of transgene ex pression in plants. Work with MARs in plants is in its early stage .In the prese nt paper ,we reviewed the procedure to isolate and identify MAR sequences from higher plants, the sequence characteristics of the plant MARs and the effect of MARs on the transgene expression in plants. Funthermore, the possible mechanism to explain how MARs affect transgene expression in transformants was discussed.
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| 关 键 词： | 基质附着区 转基因表达 Keywords 植物 matrix attachment regions（MARs） plant |
| 文章编号： | 0253-9772(2001)03-0281-04 |
| 修稿时间： | 2000-07-12 |
MARs in Plants and TheirEffects on the Transgene Expression |
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| ZHAO Yan ,,GAO Zhen yu ,HUANG Da nian. MARs in Plants and TheirEffects on the Transgene Expression[J]. Hereditas, 2001, 23(3): 281-284 |
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| Authors: | ZHAO Yan GAO Zhen yu HUANG Da nian |
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| Affiliation: | ZHAO Yan 1,2,GAO Zhen yu 3,HUANG Da nian 3 |
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| Abstract: | DNA sequences called matrix attachment regions (MARs) have recently attracted much attention because of their perceived capacity to increase levels of transgene expression and to reduce transformant-to-transformant variation of transgene expression in plants. Work with MARs in plants is in its early stage .In the present paper ,we reviewed the procedure to isolate and identify MAR sequences from higher plants, the sequence characteristics of the plant MARs and the effect of MARs on the transgene expression in plants. Funthermore, the possible mechanism to explain how MARs affect transgene expression in transformants was discussed. |
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| Keywords: | plant matrix attachment regions(MARs) transgene expression |
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