Elongation factor 2 from Artemia salina embryos and its affinity for ribosomes |
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| Authors: | M Zamboni M Brigotti L Montanaro S Sperti |
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| Affiliation: | Dipartimento di Patologia sperimentale dell'Università di Bologna, Italy. |
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| Abstract: | Crude extracts from Artemia salina undeveloped embryos do not contain detectable elongation-factor-2 (EF2) kinase and endogenous ADP-ribosylating activities. Accordingly, EF2 purified from this source is an enzyme relatively free from phosphorylated and ADP-ribosylated forms. Endogenous ADP-ribosyltransferase activity appears only after purification of EF2. The affinities of EF2 and of ADP-ribosyl-EF2 for ribosomes from A. salina undeveloped embryos have been calculated by measuring the ability of the factors to inhibit the N-glycosidase activity of ricin on ribosomes. |
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