Effect of Various Elicitors on Lignan Biosynthesis in Callus Cultures of Linum austriacum |
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Authors: | Vardapetyan H R Kirakosyan A B Oganesyan A A Penesyan A R Alfermann W A |
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Institution: | (1) Department of Biophysics, Yerevan State University, ul A. Manukyana 1, Yerevan, 375049, Armenia;(2) Institute of Molecular Biology and Plant Developmental Biology, Heinrich-Heine University, Düsseldorf, Germany |
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Abstract: | Effects of elicitors (mannan, -1,3-glucan, and ancymidol) on the activity of several key enzymes participating in lignan biosynthesis were studied in Linum austriacum L. cell cultures. The activities of L-phenylalanine ammonia-lyase, polyphenoloxydase, tyrosine ammonia-lyase, soluble phenoloxidase, and membrane-bound and soluble oxidases were assayed. The elicitors under study affected various steps in the metabolic pathway of lignan biosynthesis. Elevated enzyme activity accompanied an elicitor-enhanced synthesis of podophyllotoxins and peltatins. |
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Keywords: | Linum austriacum callus culture elicitors biosynthesis peltatins podophyllotoxins enzyme activities |
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