Increased Phosphorylation of Vimentin in Noninfiltrative Meningiomas |
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| Authors: | Ali Bouamrani Claire Ramus Emmanuel Gay Laurent Pelletier Myriam Cubizolles Sabine Brugière Didier Wion Fran?ois Berger Jean-Paul Issartel |
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| Affiliation: | 1. Grenoble Institut des Neurosciences, INSERM U836, Université Joseph Fourier, Grenoble, France.; 2. Department of Neurosurgery and Pathology, Centre Hospitalier Universitaire, Grenoble, France.; 3. Ciphergen Inc., Fremont, California, United States of America.; 4. CEA, Grenoble, France.; 5. Centre National de la Recherche Scientifique (CNRS), Grenoble, France.;Griffith University, Australia |
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| Abstract: | BackgroundTissue invasion or tissue infiltration are clinical behaviors of a poor-prognosis subset of meningiomas. We carried out proteomic analyses of tissue extracts to discover new markers to accurately distinguish between infiltrative and noninfiltrative meningiomas.Methodology/Principal FindingsProtein lysates of 64 different tissue samples (including two brain-invasive and 32 infiltrative tumors) were submitted to SELDI-TOF mass spectrometric analysis. Mass profiles were used to build up both unsupervised and supervised hierarchical clustering. One marker was found at high levels in noninvasive and noninfiltrative tumors and appeared to be a discriminative marker for clustering infiltrative and/or invasive meningiomas versus noninvasive meningiomas in two distinct subsets. Sensitivity and specificity were 86.7% and 100%, respectively. This marker was purified and identified as a multiphosphorylated form of vimentin, a cytoskeletal protein expressed in meningiomas.Conclusions/SignificanceSpecific forms of vimentin can be surrogate molecular indicators of the invasive/infiltrative phenotype in tumors. |
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