Concentration estimation via curve fitting: Quantification of negative inotropic agents by using a simple mathematical method in guinea pig atria期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

按检索

Concentration estimation via curve fitting: Quantification of negative inotropic agents by using a simple mathematical method in guinea pig atria

Authors:	Email author" target="_blank">Rudolf?Gesztelyi Email author Judit?Zsuga Béla?Juhász Péter?Dér Miklós?Vecsernyés A?József?Szentmiklósi

Institution:	(1) Department of Pharmacology and Pharmacotherapy, University of Debrecen, H-4012 Debrecen, PO Box 12, Hungary;(2) Department of Pharmacodynamics, University of Debrecen, H-4012 Debrecen, PO Box 12, Hungary;(3) Department of Pharmaceutical Technology, University of Debrecen, H-4010 Debrecen, PO Box 78, Hungary

Abstract:	We created a simple method based on curve fitting in order to assess the concentration of pharmacological agonists or antagonists in the microenvironment of the receptors. We tested our method in electrically driven guinea pig left atria by estimating the concentration of N⁶-cyclopentyladenosine (CPA; A₁ adenosine receptor agonist), acetyl-β-methylcholine (muscarinic receptor agonist) and verapamil (L-type Ca²⁺ channel inhibitor) added previously to the atria in known amounts. Our results validated the fitness of the model under specified conditions. In addition, our data suggest a relatively slow elimination of CPA in isolated, practically bloodless guinea pig atrial myocardium.

Keywords:
本文献已被 PubMed SpringerLink 等数据库收录！