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A simplified procedure for the purification of large quantities of fetal bovine serum acetylcholinesterase |
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| Authors: | D De la Hoz B P Doctor J S Ralston R S Rush A D Wolfe |
| Affiliation: | 2. School of Forensic and Investigative Science, University of Central Lancashire, Preston, Lancashire, United Kingdom;3. Office of the Vice Chancellor, London South Bank University, London, United Kingdom;1. College of Geosciences, Texas A&M University, College Station, TX USA;2. Department of Physics, University of Rome “La Sapienza”, Rome Italy |
| Abstract: | A simple procedure has been developed for the large scale purification of fetal bovine serum acetylcholinesterase (AChE) (EC 3.1.1.7). The procedure involves two steps: batch adsorption of the AChE from 250 L of serum onto a procainamide affinity Sepharose 4B gel; and analytical procainamide affinity chromatography of the step-1 product. Over 100 mg of AChE was purified in 10 days to apparent homogeneity with this procedure. |
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