Improved production of an enzyme that hydrolyses raw yam starch by Penicillium sp. S-22 using fed-batch fermentation |
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Authors: | Hai-Yan Sun Xiang-Yang Ge Wei-Guo Zhang |
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Institution: | (1) The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi, 214036, JiangSu Province, P.R. China;(2) School of Biotechnology, Southern Yangtze University, 170# Huihe Road, Wuxi, 214036, JiangSu Province, P.R. China |
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Abstract: | A newly isolated strain, Penicillium sp. S-22, was used to produce an enzyme that hydrolyses raw yam starch raw yam starch digesting enzyme (RYSDE)]. The enzyme activity and overall enzyme productivity were respectively 16 U/ml and 0.19 U/ml h in the batch culture. The enzyme activity increased to 85 U/ml by feeding of partially hydrolyzed raw yam starch. When a mixture containing partially hydrolyzed raw yam starch and peptone was fed by a pH-stat strategy, the enzyme activity reached 366 U/ml, 23-fold of that obtained in the batch culture, and the overall productivity reached 3.4 U/ml h, which was 18-fold of that in the batch culture. |
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Keywords: | Fed-batch culture Partially hydrolyzed raw yam starch Penicillium sp Peptone pH-stat Raw yam starch digesting enzyme |
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