Xanthan gum: an economical substitute for agar in plant tissue culture media |
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Authors: | R Jain S B Babbar |
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Institution: | (1) Department of Botany, University of Delhi, Delhi, 7, India |
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Abstract: | Xanthan gum, a microbial desiccation-resistant polysaccharide prepared commercially by aerobic submerged fermentation from
Xanthomonas campestris, has been successfully used as a solidifying agent for plant tissue culture media. Its suitability as a substitute to agar
was demonstrated for in vitro seed germination, caulogenesis and rhizogenesis of Albizzia lebbeck, androgenesis in anther cultures of Datura innoxia, and somatic embryogenesis in callus cultures of Calliandra tweedii. Culture media used for eliciting these morphogenic responses were gelled with either 1% xanthan gum or 0.9% agar. Xanthan
gum, like agar, supported all these responses. |
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Keywords: | Agar-agar Plant tissue culture media Xanthan gum |
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