Abstract: | The acutestimulation of glucose uptake by insulin in fat and muscle cells isprimarily the result of translocation of facilitative glucosetransporter 4 (GLUT-4) from an internal compartment to the plasmamembrane. Here, we investigate the role of SNAP23 (a 23-kDa moleculeresembling the 25-kDa synaptosome associated protein) in GLUT-4translocation and glucose uptake in 3T3-L1 adipocytes. Microinjectionof a polyclonal antibody directed to the carboxy terminus of SNAP23inhibited GLUT-4 incorporation into the membrane in response toinsulin, whereas microinjection of full-length recombinant SNAP23enhanced the insulin effect. Introduction of recombinant SNAP23 intochemically permeabilized cells also enhanced insulin-stimulated glucosetransport. These results indicate that SNAP23 is required forinsulin-dependent, functional incorporation of GLUT-4 into the plasmamembrane and that the carboxy terminus of the protein is essential forthis process. SNAP23 is therefore likely to be a fusion catalyst alongwith syntaxin-4 and vesicle-associated membrane protein (VAMP)-2.Furthermore, the endogenous content of SNAP23 appears tobe limiting for insulin-dependent GLUT-4 exposure at the cell surface.A measurable fraction of SNAP23 was sedimented with cytoskeletalelements when extracted with Triton X-100, unlike VAMP-2 andsyntaxin-4, which were exclusively soluble in detergent. We hypothesizethat SNAP23 and its interaction with the cytoskeleton may be targetsfor regulation of GLUT-4 traffic. |